Let me first describe the background of my experiment.
I study the microbiota in insects. One of my projects is to sequence the insect bacteria at different ages. The problem is everytime I have to sacrifice the insect to get the DNA for sequencing. I was wondering if I can use the longitudinal volatility function to trace the group change during the ages?
I found that there are a lot of different longitudinal functions available, do you have any recommended longitudinal function to analyze the microbiota change over time for those samples need to be sacrificed every sampling?