Hello friends-
I have just been assigned the bioinformatics part of a study after someone left their position.
My end goal is to classify sequences in samples that used ANML primers for COI.
After trimming primers I looked at the output quality plots and min sequence length:
Is it weird that the min sequencing length is so different between F and R? Ive don't this analysis twice with my own projects- and I have never had this issue.
I believe it is impacting my dada2 downstream- I am getting
Plugin error from dada2:
An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
I have tried many different trunc lengths- but I suppose that is an issue to tackle later down the line?
I am using qiime2-2019.10 because that is what my classifier was trained on.
Thanks for help!