ben
September 26, 2019, 2:58pm
2
Hi @FrancineMarques ,
Thanks for searching through the forum first and providing us in depth detail about your situation. Really helps with the troubleshooting!
This is a common enough scenario so let's take a closer look. Your dada2 stats summary show that your major loss occurs at the initial filtering step. Denoising/merging etc all look good! So, moving backwards one step, looking at your demux.qzv quality plots, it looks as though your reads are unfortunately not high in quality. There is …
Maybe this can help? it looks like you have sequences on input, but the quality plots look suspect. Is there proper overlap between the forward and reverse reads?