I finished my “alpha-rarefaction.qzv” by using “qiime diversity alpha-rarefaction”. The observed_otus arrived at a horizon at 60-100. According to other papers, this number of observed_otus is too low.

this is my feature_table summary

# Table summary

Number of samples |110|

Number of features |4,594|

Total frequency |1,498,609|

# Frequency per sample

Minimum frequency |4,998.0|

1st quartile |9,167.5|

Median frequency |13,016.0|

3rd quartile |16,425.5|

Maximum frequency |25,567.0|

Mean frequency |13,623.718181818182|

# Frequency per feature

Minimum frequency |1.0|

1st quartile |42.0|

Median frequency |107.0|

3rd quartile |284.75|

Maximum frequency |23,618.0|

Mean frequency |326.2100565955594|

My feature_table is normal? But how can I get so few observed_otus???