I am new to metagenomics data analysis.
Currently I am working on nifH amplicon data.
I have done quality filtering, reads merging, denoising and ASV construction using DADA2 denoising plugin in QIIME2.
Now I have to do taxonomic assignment to ASV’s. Currently there is no QIIME compatible sequence and taxonomy reference database available for nifH functional gene.
There’s a nifH gene sequence database constructed by jzehr lab which is in .arb format. My question is how to use such kind of databases which are in arb format to assign taxonomy. Is there any specific tool or method?
Has anyone used this database for taxonomic assignment?
Can anyone suggest me or guide me as to how can I do taxonomic assignment for nifH amplicon data ?
How can I construct a QIIME compatible reference sequence and taxonomy database for nifH gene?
Apologies if I have posted a wrong topic, I have seen some already discussed topics related to this topic…but couldn’t find any specific solution. I am asking here to get some suggestions and guidance from people who have already done such kind of work with nifH data.
Thanks in advance.