Silva in Qiime2-2020.8

[Nicholas_Bokulich]

Hello @Marisa_Tello_Martin!

@Marisa_Tello_Martin

Any idea what i am doing wrong?

Nothing! Classifiers trained on SILVA have much higher memory demands than Greengenes, being a much larger database. See this post for troubleshooting advice:

Feature-classifiers error

This is a memory error; essentially you do not have enough memory to open the SILVA classifier on your computer. This is low for the SILVA classifier — it will often take up to 32GB+ if left to its own devices! There's a couple of things you might be able to do. First, you can use the --p-reads-per-batch parameter (e.g., set to 1000 or 2000) to reduce the number of reads classified at a time. However, it looks like you do not have enough memory to load the SILVA classifier, not the reads, s…

Good luck!