Sorry if this has already been posted in the forum that I missed, but I had a question regarding the representative sequences that are generated from the dada2 denoising step. When I create a visualization of the rep-seqs.qza file, I noticed that the sequences are not all the same length. When I had performed this function on other datasets, I thought that the rep-seqs that were generated were all the same length. Is it normal for the representative sequences to have variation in length, or is there something that I performed incorrectly?
The code I used is listed below:
qiime dada2 denoise-paired
I am performing this on Illumina Miseq 2 x 151nt run on the 16s region, that was imported through the Earth Microbiome Project format for more information.