I then use the feature classifier to assign taxonomies:
qiime feature-classifier classify-consensus-blast
--i-query mifish_rep-seqs.qza
--i-reference-reads references.12s.miyaSeqs.qza
--i-reference-taxonomy references.12s.miyaTaxonomy.qza
--p-perc-identity 0.97
--output-dir references.12s.miyaTaxonomy97
But I always get an error for any new database I try:
Plugin error from feature-classifier:
BLAST6 file is empty.
Could you please help me out? This used to work really well, maybe 3-4 years ago but not anymore.
Thank you.
Vera
I've checked and that might be the reason. thanks.
another error with a different data and database shows up.
When I run :
qiime feature-classifier classify-consensus-blast
--i-query nis_trimmed_ASVs/nis_rep-seqs.qza
--i-reference-reads COIsequence23.qza
--i-reference-taxonomy COItaxonomy23.qza
--p-perc-identity 0.97
--output-dir nis_COItaxonomy97/
--verbose
I get this error: (1/1) Invalid value for '--i-reference-reads': COIsequence23.qza was created by 'QIIME 2023.5.1'. The currently installed framework cannot interpret archive version '6'.
It looks like COIsequence23.qza was generated with qiime 2023.5, which introduced a new archive version, but you're using something lower than 2023.5. If you upgrade to 2023.5 or generate COIsequence23.qza with <2023.5, this issue should go away.