Code: qiime brocc classify-brocc --i-query query.qza --o-classification query-brocc.qza
Result:
Plugin error from brocc:
Command '['blastn', '-query', '/tmp/qiime2/xiaoe/data/50c33ae5-dfe9-4b83-a607-8579e0a27090/data/dna-sequences.fasta', '-evalue', '1e-05', '-outfmt', '7', '-db', 'nt', '-max_target_seqs', '100', '-out', '/tmp/tmp14oivpy7']' returned non-zero exit status 2.
Debug info has been saved to /tmp/qiime2-q2cli-err-9vexhi0r.log
question:Can anyone tell me how to fix this, I'm using the sample file provided on the brocc website?
Second
I want to annotate marker genes of archaea, at the moment I don't know how to do it, so I used brocc's method, is there any other way than that, thanks.
BLAST Database error: Could not find volume or alias file (nt.01) referenced in alias file (/mnt/f/blast/nt/nt).
Traceback (most recent call last):
File "/home/xiaoe/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/q2cli/commands.py", line 339, in __call__
results = action(**arguments)
File "<decorator-gen-470>", line 2, in classify_brocc
File "/home/xiaoe/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/qiime2/sdk/action.py", line 234, in bound_callable
outputs = self._callable_executor_(scope, callable_args,
File "/home/xiaoe/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/qiime2/sdk/action.py", line 381, in _callable_executor_
output_views = self._callable(**view_args)
File "/home/xiaoe/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/q2_brocc/plugin_setup.py", line 59, in classify_brocc
subprocess.run(blast_cmd, check=True)
File "/home/xiaoe/miniconda3/envs/qiime2-2022.8/lib/python3.8/subprocess.py", line 516, in run
raise CalledProcessError(retcode, process.args,
subprocess.CalledProcessError: Command '['blastn', '-query', '/tmp/qiime2/xiaoe/data/50c33ae5-dfe9-4b83-a607-8579e0a27090/data/dna-sequences.fasta', '-evalue', '1e-05', '-outfmt', '7', '-db', 'nt', '-max_target_seqs', '100', '-out', '/tmp/tmpz5lsxcev']' returned non-zero exit status 2.
