Currently manually installing PICRUSt2. Downloaded install file from https://github.com/gavinmdouglas/q2-picrust2 - version 2019.10.
Running in Conda environment version 4.9.2
Python version: 3.8.3
Following code worked well:
conda install -c bioconda -c conda-forge picrust2
cd picrust2-file-downlaod
pip install -e .
When I go do to:
conda dev refresh-cache
The following error is returned:
(qiime2-2020.8) z_m@x86_64-apple-darwin13 ~ % qiime dev refresh-cache
QIIME is caching your current deployment for improved performance. This may take a few moments and should only happen once per deployment.
Traceback (most recent call last):
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/bin/qiime", line 11, in <module>
sys.exit(qiime())
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/click/core.py", line 829, in __call__
return self.main(*args, **kwargs)
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/click/core.py", line 782, in main
rv = self.invoke(ctx)
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/click/core.py", line 1259, in invoke
return _process_result(sub_ctx.command.invoke(sub_ctx))
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/click/core.py", line 1259, in invoke
return _process_result(sub_ctx.command.invoke(sub_ctx))
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/click/core.py", line 1066, in invoke
return ctx.invoke(self.callback, **ctx.params)
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/click/core.py", line 610, in invoke
return callback(*args, **kwargs)
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/q2cli/builtin/dev.py", line 31, in refresh_cache
import q2cli.core.cache
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/q2cli/core/cache.py", line 406, in <module>
CACHE = DeploymentCache()
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/q2cli/core/cache.py", line 61, in __init__
self._state = self._get_cached_state(refresh=refresh)
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/q2cli/core/cache.py", line 107, in _get_cached_state
self._cache_current_state(current_requirements)
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/q2cli/core/cache.py", line 200, in _cache_current_state
state = self._get_current_state()
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/q2cli/core/cache.py", line 238, in _get_current_state
plugin_manager = qiime2.sdk.PluginManager()
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/qiime2/sdk/plugin_manager.py", line 54, in __new__
self._init(add_plugins=add_plugins)
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/qiime2/sdk/plugin_manager.py", line 81, in _init
plugin = entry_point.load()
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/pkg_resources/__init__.py", line 2472, in load
return self.resolve()
File "/Users/z_m/opt/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/pkg_resources/__init__.py", line 2478, in resolve
module = __import__(self.module_name, fromlist=['__name__'], level=0)
File "/Users/z_m/Desktop/q2-picrust2-2019.10_0/q2_picrust2/__init__.py", line 9, in <module>
from ._full_pipeline import full_pipeline
File "/Users/z_m/Desktop/q2-picrust2-2019.10_0/q2_picrust2/_full_pipeline.py", line 7, in <module>
from picrust2.default import (default_ref_dir, default_tables,
ImportError: cannot import name 'default_ref_dir'
I can’t even initialize QIIME2 now without throwing this error. Anyone know how I resolve this?
