Hi @cindy_Johanna_Nino_C,
picrust2 is not part of QIIME 2 so we are not able to provide support for that on this forum. If you are using the QIIME 2 plugin q2-picrust that is sort of another story...
You also opened up this related topic:
so please stick with one topic or the other and decide whether you plan to use picrust2 or q2-picrust2.