I’m processing data to compare microbial community shifts and I’ve imported a file with my data filtered for cyanobacteria only and updated the taxonomy; however, when I try to run pairwise PERMANOVA I get an error (see below). I don’t receive this error when running pairwise PERMANOVA’s with the original, unfiltered file. All files are formatted the same with the exception of removing non-cyanobacteria taxa.
Plugin error from diversity:
All values in the grouping vector are unique. This method cannot
operate on a grouping vector with only unique values (e.g., there are
no ‘within’ distances because each group of objects contains only a
Debug info has been saved to /tmp/qiime2-q2cli-err-jb1gnjr1.log.
I would appreciate any help! Thanks!