I am about to send samples of extracted DNA out to a lab for library preparation and sequencing. I will be using the 515f-806r primers for the V4 region. I am unsure whether I should use 2x151 or 2x250 paired end sequencing for this region.
The sequencing strategy used by the lab I am working with does not include the primers in the reads. From a previous post it seems like the 2x151 sequencing will be sufficient to merge reads if the primers aren’t included in sequencing but I wanted to double check since this work is quite new to me.
Thank you for your help,