Number of basepair to trim in DADA2?

General Discussion
1

Hello everyone,

I am new to qiime2 and I need help with deciding the trim value for DADA2.

The length of my forward primer (ITS5) is 22 and of reverse primer (ITS2) is 20. Also the length of barcode is 6. I have also uploaded the interactive plot visualization. It would be great if you all can help me in understanding and deciding the number of nt to trim.

Thank you in advance

screencapture-file-tmp-qiime2-archive-_9e7glrb-b34e3fed-b33f-4bd6-8211-909105cd99ed-data-index-html-2018-12-17-18_42_48
screencapture-file-tmp-qiime2-archive-_9e7glrb-b34e3fed-b33f-4bd6-8211-909105cd99ed-data-index-html-2018-12-17-18_42_481853×1637 361 KB

2

See here for a guide straight from the source.

Your sequence quality looks good — you can truncate just the last few nucleotides off of each end.

3

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