I am not getting any sequence info from the demultiplexed .qzv files.
Any Idea what is the possible error?
I am not getting any sequence info from the demultiplexed .qzv files.
Welcome to the forums, Noman!
That table is interactive! If you mouse over the plot, the table will automatically update with statistics from that position in the read.
Let us know if you have more questions!
Thanks, Colin! Yes, that's the issue. It shows the same quality score for all the sequence base positions, which feels like all the sequences are the same. I am not sure if there is anything wrong with the data formatting. I have uploaded a picture of how the data is formatted. I am also adding the .qzv file for your reference if you can suggest anything. Thank you very much!
chapter3.zip (284.6 KB)
Yes! These are probably Illumina's new 'binned' quality scores. They can cause issue with DADA2, sometimes.
I would continue with cautious optimism and see how it goes!
which feels like all the sequences are the same
That would be an issue, and you could see that during denoising or during taxonomy assignment. Or maybe it's fine.