Hi, I am trying to demultiplex my qza, which has the barcodes in it. The datademux.qza is a Multiplexed single end barcode sequence.
So I do the following:
qiime cutadapt demux-single --i-seqs datademux.qza --m-barcodes-file mapping_file_ARCH.tsv --m-barcodes-column BarcodeSequence --p-error-rate 0 --o-per-sample-sequences demultiplexed-seqs.qza --o-untrimmed-sequences untrimmed.qza --verbose
And I get an error. I printed screen the commands. Any clue why I am getting this error?
Hi, i am trying to demux (q2 2020.2) my file imported from ion torrent fastq data and I put this command:
qiime cutadapt demux-single
and I get this command problem:
Invalid value for “–i-seqs”: Expected an artifact of at least type
MultiplexedSingleEndBarcodeInSequence. An artifact of type
SampleData[SequencesWithQuality] was provided.
Any idea why I am getting this error? And which command should I use?