cutadapt demux-single error

Technical Support
1

Hi, i am trying to demux (q2 2020.2) my file imported from ion torrent fastq data and I put this command:

qiime cutadapt demux-single
–i-seqs data_demux.qza
–m-barcodes-file barcode.tsv
–m-barcodes-column barcode
–p-error-rate 0
–o-per-sample-sequences demultiplexed-seqs.qza
–o-untrimmed-sequences untrimmed.qza
–verbose

and I get this command problem: :frowning_face:

Invalid value for “–i-seqs”: Expected an artifact of at least type
MultiplexedSingleEndBarcodeInSequence. An artifact of type
SampleData[SequencesWithQuality] was provided.

Any idea why I am getting this error? And which command should I use?

Thanks :smile: :smile:

2

2 posts were merged into an existing topic: No samples found demultiplexing error