More sequences were provided than barcodes

User Support
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1

Hi All,

I am looking for some insightful answers to solve this problem:

Plugin error from demux:
More sequences were provided than barcodes.

If someone can help me, I appreciate it.

FL

2

Hello and welcome to :qiime2:!

From the available information I cannot really understand what is happening, you need to look at your data. There are 2 possibilities:
[EDIT]

  1. There are more sequences than barcodes in your data, it's easy to check with the notebook.
  2. The import was incorrect - check if your sequencing data is of the correct type and was imported correctly.

Cheers
V

3

In this case, it's the opposite. It declares that there are more sequences than barcodes. My metadata file with the barcode contains 67 samples. So, for me, it sounds like I can't retrieve the sequences from my original file using a shorter number of barcodes (e.i : I have 100 barcoded samples and I only want to demultiplex 40 of them).
Could I explain you well?

4

Sorry, I corrected the post before, it was a typo.

For QIIME2 to properly import the data number of barcodes should be equal to the number of sequences.

Cheers,
V

5

Hi @crusher083 ,
So it means that, in my sequence file, I can not have sequences barcoded with barcodes not present in my metadata?

Cheers,
FL

6

Yes, that is correct. You may filter them out beforehand.

Cheers
V

7

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