I’m Restu, a Ph.D. student from UQ, also a newbie in microbiome analysis. Hopefully, this question is valid.
Currently, I’m still using QIIME 1.9, but I’m planning to to migrate my pipeline to QIIME 2 per this forum suggested. (al so interested to try DADA2)
Just a little bit confused when in the QC steps. OK this is my data:
my data is demultiplexed cassava paired end :
I guess I’ve read how to import it in the importing tutorials, but the problem is, the imported files still don’t have the sample name…
I usually do this in qiime 1:
split_libraries_fastq.py -i J612_S48_L001/fastqjoin.join.fastq -o sample_reads/R0078 --sample_ids R0078 -q 19 --barcode_type ‘not-barcoded’
is there any way to assign the sample name during the “import” like split_libraries_fastq.py’s --sample_ids ???
And how to make the imported file ready for DADA2 ?
Thank you very much,