Many unassigned features using SILVA trained classifier for V1-V3 hypervariable region

Assuming your reads are not in a mixed orientation (you can search the forum for more threads on this issue), I'm not sure what else to suggest here.

But as I mentioned earlier, if all the other regions look okay, then it may be that V1V3 region does not contain the resolution needed for your study system. Perhaps manually BLAST a few sequences to be sure? :man_shrugging: