I am working on 16S rRNA gene V4 region analysis using QIIME2 2024.10 on a Windows machine with WSL (16GB RAM). I am looking for a pre-trained SILVA 138 99% OTU classifier for the V4 region with the following specifications:
My computer has 16GB RAM and is unfortunately unable to train the classifier locally due to memory limitations. The process gets killed before completing.
If anyone has this classifier file (silva-v4-classifier.qza) or knows where I can download a compatible pre-trained version, I would really appreciate your help!
I have one trained with the same primers (just checked) and Qiime2 version, Silva 138.2 (did you mean the first release 138 with outdated taxonomy? Mine is trained on 138.2, with updated names).
Here is the link. All files are in the q2-2024-10/Silva138_2 directory, with V4 in the name (classifier, taxonomy and sequences).
Thanks for your help and suggestions. This is helpful. I need to use Silva; I found one pretrained Silva 138 classifier in Github and I see one more reply here in which a pretrained version is shared-I will try using that. Thanks again for your time and sharing information.
We are still waiting to hear back from Silva on this, but we are hoping that it will be quite soon. In the meantime, you can either train your own classifier (@SoilRotifer has a great tutorial for this outlined here) or you can use the one(s) that @timanix trained. We will make sure to let everyone know once Silva has made the new classifiers available!
