ITSxpress Plugin error: "Missing sequence for record beginning on line 9"

[colinbrislawn (Colin J Brislawn)]

Hello João,

Welcome to the forums!

There is a number of things that can cause that error. This old post from Chris is a good place to start:

Plugin error from itsxpress: fastq.gz is not a(n) FastqGzFormat file and Missing sequence for record beginning on line 13

Welcome to the forum, @Yujia! A quick search shows that Missing sequence for record beginning on line 13 is an error message that pops up with some frequency. Here are two posts that cover the issue: The first one suggests you make sure your version of itsexpress is up-to-date (at least version 1.7.2). It sounds like you've tried to do that, but it's worth checking exactly what version you have before moving on. 1.7.2 should fix this problem for you. If that doesn't work, the second tac…

@Joao_Nuno

• How the data was generated: Illumina Nextseq 2000 P1 600 cycles

This is also helpful, because it can help calculate the expected amplicon coverage, if you know the of expected amplicon length (distribution, because there are lots of amplicons). But check the other things first!

Let us know what you try next and if you need more help!