I run Deblur on my data (single-end reads generated using Illumina Miseq) and then created two artifacts containing summary statistics using this block of commands:
qiime metadata tabulate
qiime deblur visualize-stats
This is the results:
1- Is it weird to get ZERO for reads-too-short-after-truncation and reads-exceeding-maximum-ambiguous-bases? How about reads-truncated? Could it be because my data had been quality filtered before importing into QIIME2?
2- What is the most important column of the second table (second screenshot) that I need to take into account before proceeding with the next step of my analysis? What is reads-hit-reference? what is reference here?
3- What is reads dereplication?
Sorry for asking basic questions and thank you for creating this amazing forum that is helping me through my first project.