Input Error Assistance

[SoilRotifer (Mike Robeson)]

Hi @Megan_Whisonant welcome to !

This issue is that the sequence file names violate the CASAVA naming schema as outlined here. I'd recommend using the Manifest format.

See these related posts:

importing demultiplexed fastq.gz files without barcode identifier in filenames

This is because that format has a specific naming schema, as outlined here. Also, see:

Problem importing fastq.gz files after quality Trimming with cutadapt

Hi @sleva88, welcome to ! I think the names of your files, e.g. C1_S43_L001_R1_001_trimmed.fastq end up violating the naming schema for Casava. I think if you remove _trimmed from the file names it should work. Alternatively, you can leave the files named as they are and try the Manifest format. -Mike

Tutorial - Data imports

Okay, so you have a folder, on your local computer, full of sequence files already separated out by sample. Or do you have one single sequence file? Is it a QZA file? ZIP file? Something else? Assuming you have a folder full of separate sequence files, it sounds like you have everything you need to import your data. You should be able to import the data following Manifest approach. Alternatively, you might also be able to import via CASAVA approach, assuming your file names fit that schema (s…

-Mike