I a college student, new to Qiime2, that is utilizing this platform for undergraduate research. Version of Qiime2: 2020.2.0
Command and Error Message:
student@ubuntu1804:~/MeganW$ qiime tools import \
--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path fasta-qual-mapping-files
--input-format CasavaOneEightLanelessPerSampleDirFmt
--output-path fasta-qual-mapping-files.qza
There was a problem importing fasta-qual-mapping-files:
Missing one or more files for CasavaOneEightLanelessPerSampleDirFmt: '.+_.+_R[12]_001\.fastq\.gz'
For reference purposes, this is how my mapping files look, which is why I used the LaneLess option.
Hi @Megan_Whisonant welcome to !
This issue is that the sequence file names violate the CASAVA naming schema as outlined here. I'd recommend using the Manifest format.
See these related posts:
-Mik
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