Input Error Assistance

I a college student, new to Qiime2, that is utilizing this platform for undergraduate research. Version of Qiime2: 2020.2.0

Command and Error Message:
[email protected]:~/MeganW$ qiime tools import \

--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path fasta-qual-mapping-files
--input-format CasavaOneEightLanelessPerSampleDirFmt
--output-path fasta-qual-mapping-files.qza

There was a problem importing fasta-qual-mapping-files:

Missing one or more files for CasavaOneEightLanelessPerSampleDirFmt: '.+_.+_R[12]_001\.fastq\.gz'

For reference purposes, this is how my mapping files look, which is why I used the LaneLess option.

Hi @Megan_Whisonant welcome to :qiime2:!

This issue is that the sequence file names violate the CASAVA naming schema as outlined here. I'd recommend using the Manifest format.

See these related posts:

-Mik

3 Likes

Thank you very much!