Importing paired-end de-multiplexed fastq files into qiime2 as .qza artifact

[Nicholas_Bokulich]

Welcome to the forum @noor_uto!

Glad you got the import issue sorted out; we will assist you with your dada2 issue in this separate topic:

Errors while utilizing dada2 for denoise-paired for demultiplexed data

Hello, I am using qiime2 2020.2, I have successfully imported my fastq.gz files in one QIIME2 artifact named paired-end-demux.qza. I am attempted to utilize the dada2 denoise-paired command but I am running into issues. My command was as follows: >qiime dada2 denoise-paired --i-demultiplexed-seqs paired-end-demux.qza --p-trunc-len-f 244 --p-trunc-len-r 200 --output-dir dada2_output/ Plugin error from dada2: No reads passed the filter. trunc_len_f (244) or trunc_len_r (200) may be individuall…

(thanks for opening your new question in a separate topic, but please do not cross-post as you have done here. Thanks!)