I would like to analyse 454 data using QIIME 2, particularly would like to use the denoise-pyro method of DADA2.
I have experience working with MiSeq data but I am still struggling to get going with 454 data that I was given. I have a single .fna and respective .qual file from the sequencing run, and I was wondering if I can get some help about how to import these to QIIME2, in a format that I can use DADA2.
Hi @Mdavrandi,
QIIME 2 does not have any methods to handle separate fasta and qual files. You will need to convert to fastq with another program, e.g., this QIIME 1 script.
Thanks @Nicholas_Bokulich. Thats exactly where I got stuck, though I finally managed to work it out by doing the following: Demultiplexed data in QIIME 1;
I was able to run DADA2 denoise-pyro after going through this. I am not sure if this is ideal or not but it worked. I appreciate if you could point out if there is a better way of doing this.