I have had trouble being able to process my data with dada2.

[cinthya_vieyra]

Good afternoon
I have had trouble being able to process my data with dada2. I hope it can help me because I don't know how to continue or what to change.
I am using virtual box, version 2020.11 of QIIME2.
I send you a screenshot with the error.
Thank you so much for your help!

[andrewsanchez]

Hi @cinthya_vieyra,

If you search the forum for something like "virtualbox error was encountered while running dada2," you will find that this issue has been discussed before. For example, here you will find a nice suggestion for solving the issue:

An error was encountered while running DADA2

@ptalebic, this isn’t an issue I’ve dealt with personally before, but I’m sure you can figure it out by trial and error. Remember, if you’re running 64-bit Windows 10, that the host OS needs at least 2GB of dedicated RAM to work smoothly. Virtualbox allocates memory in MB (1024 MB is ~1GB of memory), and your Box’s 1GB of RAM is probably not enough for DADA2 to run smoothly - try taking a look at how much memory your system has available, and bumping up your VirtualBox allocation without starvi…

Let us know if you need any help after following the advice in other discussions!

Cheers,
Andrew