Hello! I apologize if I use the wrong words. I am recently working on data analysis and I have some questions. How can I classify my ASVs with a sequences reference database? When obtaining ASVs, is it possible to perform a grouping by reference to 99% identity to obtain OTU's?. And if it is, wi…

Hi @cinthya_vieyra , ASVs are the highest resolution version of your sequences, think of them as 100% OTUs. If you want to cluster them using a reference database (ex Greengenes, Silva, GTDB etc.) you can certainly do that using q2-vsearch, see this tutorial for more details. My recommendation is to…

Thank you so much for your help!

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How can I classify my ASVs with a sequence reference database?

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