According the rule of forum, we searched and found this related link (Red interactive quality plot - #3 by thermokarst). However, there is no intepretation on interaction plot like that.
How can we set the parameter on next step denoise-paired?
Thank for your help!
Hi @Wang_cs001632,
This is a rather odd looking QC plot. What was the sequencing equipment that produced this? And more importantly, what modifications were made to the reads prior to importing to QIIME 2?
For some reason, it looks to me as though each of your Forward and Reverse sets are made up of 2 separate ~150 bp runs that have been merged. Is this a possibility?
Could you also provide us with additional info like what target region and primers is this from? Expected overlap region?
This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.