How to convert BAM format to Fastq format?

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1

Hello,

I got seq data from PacBio plateform. That was formatted by BAM. Do you know how to convert xx.bam file to fastq file to analyze them in qiime2?

Thanks,

Hee-Sung

2

Hi @baehsung,
Looks like you can use the command bamtofastq. Here is the link to the documentation: bamtofastq — bedtools 2.31.0 documentation

Hope that helps!
:turtle:

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3

Hello!

Looks like PacBio also has their own software for converting their BAM files into Fastq files.

See this PDF, page 3, bam2fastq

bam2fastq -o myEcoliRuns \
  m54008_160330_053509.subreads.bam \
  m54008_160331_235636.subreads.bam
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4

Dear Hee-Sung

You can also use samtools to convert BAM or CRAM to either FASTQ or FASTA files.
It's a useful tool alongside the other recommendations.

5

Thanks all for kind replies.

SHKose,
I run qiime2 in miniconda environments. Do you know the codes how to download samtools and install it in my system?

Hee-Sung

6

Dear Hee-Sung,

You'll be able to install it within your conda environment with the following commands:

conda install -c bioconda samtools
conda install -c "bioconda/label/cf201901" samtools

https://anaconda.org/bioconda/samtools

7

Dear @baehsung @skose82 ,

Depending on what plugins you installed in your conda environment, samtools should already be installed. It is a dependency for a few QIIME 2 plugins, including at least one in the standard core distribution I believe. So try running this to check your environment before installing:

conda list samtools

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