how to compute alpha diversity and beta diversity without rarefaction

[colinbrislawn (Colin J Brislawn)]

Hello Wei_Zhang,

You are correct, there is a tradeoff between keeping more samples and keeping more depth, which has been discussed here and here. I don't think there's a perfect solution.

@Wei_Zhang

I wonder whether there is an alternative way to normalize the data

I have not tried this, but you could try the SRS tool!