fq-manifestor: a web app for generating fastq manifest files

Hello :qiime2: Forum! I'd like to share fq-manifestor, a little web utility to help with creating fastq manifest files from paired-end Illumina sequencing data. You can find this here.

This allows you to drag-and-drop your folder containing fastq files[1], and it will attempt to create a fastq manifest file for use in importing data into QIIME 2. It uses regular expressions to try to identify information from the file names, including the sample ids and which files represent the forward and the reverse reads for each sample. The patterns can be modified through the web app, which will update a preview so you can experiment if your filenames don't align with the default patterns.

Here's a little video that shows how it works.

Happy manifesting! :fire::fire:


  1. Importantly, your data stay on your computer - nothing is uploaded. The drag-and-drop step simply allows for fq-manifestor to get the list of files found under the directory you dropped. â†Šī¸Ž

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