Hi @Priyanka_Mishra,
Your demux file looks fine and your provenance makes sense to me. The last think that I can think of that might be effecting overlap is your adapters are still in the sequences? Do you know if your adapters are still in the sequence? If they are, you will want to use cutadapt to trim them out before dada2. Demultiplexing and Trimming Adapters from Reads with q2-cutadapt
Hope that helps!
