Hello,everyone
i used dada2 to denoise my pair-end illumina sequences (16S V4 region, primer 515F-806R). I have two samples, but finally i end up with a each feature is only present in a single sample. Like this:
I think barcode or primer have been removed, bacause I have trimed left 30 bp, the code is following:
time qiime dada2 denoise-paired --i-demultiplexed-seqs demux.qza --p-trim-left-f 30 --p-trim-left-r 30 --p-trunc-len-f 190 --p-trunc-len-r 190 --o-representative-sequences rep-seqs.qza --
o-table table.qza --o-denoising-stats stats.qza
Does anybody know how to solve this?
Thanks very much!!