Cross-referencing another post here. @Mechah provided some great discussion of his lab's protocols for contaminant removal from low-biomass samples in this topic:
Hi Jia,
sorry for my late reply due to the Christmas break…
How to handle controls in our NGS projects is still a matter of debate in our lab. However, from my point of view I would suggest to do the following (I’ll only tackle data handling and won’t mention wet lab methods to get rid of the “kitome” in this post):
First of all you should process as many controls as possible. I would consider negative controls (NTCs, field blanks etc.), positive controls (DNA of mock communities), and DNA ex…
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