Demultiplexing reads without sample barcode metadata

Hi everyone,

First of all, I am sorry that I have to re-ask this in the forum. I have searched the forum for resources regarding my case and almost always got referred to this link for demultiplexing reads with barcode in the sequence and without the barcode metadata. However, the tutorial showed how to do the demultiplexing with sample barcode metadata, and qiime cutadapt demux-paired still requires forward barcode file.

Now onto my data, I have multiplexed files that I have imported to multiplex-seqs.qza format. They are paired-end reads. The next step is to demultiplex them, however, I don't have the sample barcode metadata. The barcode is in the sequence. Could someone point me to the most updated tutorial on how to do this?

Thanks in advance!

Hi @emntsha,

Unfortunately, you will need the sample barcode metadata for this workflow. Can you get in touch with the lab that performed this data prep? They should be able to help you!
Once you get that data, let me know if you need help from there.


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