Demultiplexed demux.qza to fastq files

User Support
1

Hi,
I want to use qiime2 to demultiplex R1.fastq, R2.fastq with Index.fastq and barcodes into each sample with sample1_R1.fastq, sample1_R2.fastq
Currently, I searched the forum. I have not found a function can do that. May I have the help?
Thanks.

2

This is explained in detail in the Moving Pictures tutorial, as well as the Importing tutorial - please take a peek at those for some assistance!

3

Hi, @thermokarst,

Thanks for the reply.
But what I want to get is to convert demux.qza to different samples’ R1.fastq and R2.fastq file. IS there any way to achieve it?

Thanks.

4

You can export your demultixplexed reads!

https://docs.qiime2.org/2018.11/tutorials/exporting/

5

Thanks @thermokarst. It works!

6

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