DADA2 Quality Control ERROR

[Nicholas_Bokulich]

Welcome @matthewbur!

See here:

–o-denoising stats: command not found

@farhad63, The issue is that your command is separated onto multiple lines. Put your entire command on a single line like to prevent this issue from occurring. Alternatively, put a backslash character (\) at the end of each line to let the computer know that your command continues onto the next line. You will note this is what is done in the QIIME 2 documentation — breaking up the command onto multiple lines makes it easier to read in the tutorial, but requires the backslashes to the computer …

@matthewbur

The code was working just fine earlier in the day

You probably copied/pasted from earlier and/or changed a line and forgot to add the backslash to the end of the line.

Good luck!