Hello. I am currently using Qiime2-2020.11 and began receiving an error during my quality control step for the first time.
Code:
qiime dada2 denoise-paired
–i-demultiplexed-seqs demux.qza
–p-trim-left-f 19
–p-trim-left-r 16
–p-trunc-len-f 220 \
–p-trunc-len-r 200 \
–o-table table.qza
–o-representative-sequences rep-seqs.qza
–o-denoising-stats denoising-stats.qza
Errors:
(1/5) Missing option ‘–p-trunc-len-r’.
(2/5) Missing option ‘–o-table’. ("–output-dir" may also be used)
(3/5) Missing option ‘–o-representative-sequences’. ("–output-dir" may also be used)
(4/5) Missing option ‘–o-denoising-stats’. ("–output-dir" may also be used)
(5/5) Got unexpected extra argument ( )
zsh: command not found: --p-trunc-len-r
zsh: command not found: --o-table
I am not sure why this started happening. The code was working just fine earlier in the day. Any suggestions as too what may resolve the issue is appreciated.
Thanks!