dada2 Error: (return code -9) in virtual box

[Pratibha_Kadam]

I have checked the other posts and tried to solve the issue.

DADA2 error (return code -9) and (return code 1)

Hi, I am using QIIME2 2019.1 on virtualbox. I was running DADA2 when I encountered the following errors: qiime dada2 denoise-paired \ –i-demultiplexed-seqs demux-paired-end.qza –p-trim-left-f 6 –p-trim-left-r 6 –p-trunc-len-f 200 –p-trunc-len-r 220 –o-table table-dada2.qza –o-representative-sequences rep-seqs-dada2.qza –o-denoising-stats denoising-stats.qza Plugin error from dada2: An error was encountered while running DADA2 in R (return code -9), please inspect stdout and …

But I keep getting this error even if I have added more memory. My computer is of 64GB RAM is that sufficient for the analysis ??

When I run dada2 denoise-paired, the --p-n-threads option does not take threads and runs on only 1 thread ! So, It took me 65 hours to finish the run for the 7GB input file.

[colinbrislawn (Colin J Brislawn)]

Good morning,

I'm glad you were able to find that other thread. I wonder why the --p-n-threads setting is not working for you...

Can you post the full command you ran?

[Pratibha_Kadam]

I was using older version (2020) and R in that doesn't use multithreads.
I have installed latest version and it worked for me.

Thanks for your support @colinbrislawn