Hi,
I am using QIIME2 2019.1 on virtualbox.
I was running DADA2 when I encountered the following errors:
qiime dada2 denoise-paired \
--i-demultiplexed-seqs demux-paired-end.qza
--p-trim-left-f 6
--p-trim-left-r 6
--p-trunc-len-f 200
--p-trunc-len-r 220
--o-table table-dada2.qza
--o-representative-sequences rep-seqs-dada2.qza
--o-denoising-stats denoising-stats.qza
Plugin error from dada2:
An error was encountered while running DADA2 in R (return code -9), please inspect stdout and stderr to learn more.
Debug info has been saved to /tmp/qiime2-q2cli-err-_kcduaha.log
After which I tried to run it again with different p-trim-left values.
qiime dada2 denoise-paired \
--i-demultiplexed-seqs demux-paired-end.qza
--p-trim-left-f 0
--p-trim-left-r 0
--p-trunc-len-f 200
--p-trunc-len-r 220
--o-table table-dada2.qza
--o-representative-sequences rep-seqs-dada2.qza
--o-denoising-stats denoising-stats.qza
Plugin error from dada2:
An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
Debug info has been saved to /tmp/qiime2-q2cli-err-oog8x06p.log
Does anyone know what is causing these errors?