Hi Good day. I am currently analysing 6 samples using qiime2. I previously analyzed my data using qiime 1.9.1 however, due to several papers discussing the advantage of using amplicon sequence variants instead of OTUs, i re-run my analysis as i would like to see it for my self. my data were sequenced on Illumina Miseq 300PE with the initial count below.

Sample 1 220, 051

Sample 2 213,886

Sample 3 175,420

Sample 4 143,264

Sample 5 115,665

Sample 6 96,526

after importing my paired-end sequences, I used dada2 denoised paired with the following parameters:

```
qiime dada2 denoise-paired \
--i-demultiplexed-seqs demux.qza \
--o-table table.qza \
--o-representative-sequences rep-seqs.qza \
--p-trim-left-f 0 \
--p-trim-left-r 0 \
--p-trunc-len-f 280 \
--p-trunc-len-r 280
```

However, after viewing the summary of feature table frequency (OTU table biom) and feature data sequence (rep sequences). i gives me the following counts:

no of features: 365

total no of frequency: 17,333

Sample 1- 3, 868

Sample 2 - 3,622

Sample 3 - 2,918

Sample 4 - 2,400

Sample 5 - 2,319

Sample 6 - 2,206

Is the 17, 333 the total number of ASV or sequences that has been generated by dada2? i found it reaaly small as compared with my initial analysis with qiime1 were i got 623, 435 sequences after quality filtering.

or the 17, 333 is just the total number of unique ASV that has been group together as similar with each other? if so, is there a way where i can find the total number of sequences that has generated by dada2?

thank you so much and i hope some can enlighten me.