dada2 demultiplexed-seqs error

Technical Support
1

Running qiime2.2024.2 on my institution's computing cluster (so not a memory issue) and having an issue running this bit of code:

qiime dada2 denoise-paired
--i-demultiplexed-seqs demux.qza
--p-trunc-len-f 290 \
--p-trunc-len-r 275
--output-dir dada2/
--p-n-threads 10
--verbose

It returns "Invalid option '-i--demultiplexed-seqs]' Try module --help for more information" but this is directly from the tutorial and seems to be the boilerplate first parameter? I actually ran this on my data a week ago and it had no issue, but redoing it seems to have caused a problem.

2

Hello @op9183,

Make sure you end each line except the last one with a space and then a backslash \:

qiime dada2 denoise-paired \
--i-demultiplexed-seqs demux.qza \
--p-trunc-len-f 290 \
--p-trunc-len-r 275 \
--output-dir dada2 \
--p-n-threads 10 \
--verbose
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