Hi, i am trying to demux (q2 2020.2) my file imported from ion torrent fastq data and I put this command:
qiime cutadapt demux-single
--i-seqs data_demux.qza
--m-barcodes-file barcode.tsv
--m-barcodes-column barcode
--p-error-rate 0
--o-per-sample-sequences demultiplexed-seqs.qza
--o-untrimmed-sequences untrimmed.qza
--verbose
and I get this command problem: ![]()
Invalid value for "--i-seqs": Expected an artifact of at least type
MultiplexedSingleEndBarcodeInSequence. An artifact of type
SampleData[SequencesWithQuality] was provided.
Any idea why I am getting this error? And which command should I use?
Thanks
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