Hi I am trying to cluster my ASVs into OTUs by following this Qiime Tutorial
I have tried adding my fasta file downloaded from rep-seq but I am getting this error
(1/1) Invalid value for '--i-sequences': Expected an artifact of at least
type SampleData[Sequences] | SampleData[SequencesWithQuality] |
SampleData[JoinedSequencesWithQuality]. An artifact of type
FeatureData[AlignedSequence] was provided.
Further I am not able to convert fasta file in fna for initially converting in into qza
Please assist me with this
Welcome to the forum!
Your issue is here:
You are providing aligned sequences and your command doesn't allow aligned sequences. Do you have any of these types: SampleData[Sequences] | SampleData[SequencesWithQuality] |
Can you provide more info? What command are you running and what does your data look like?
Hey, Thanks for your help.
I used the code available for Denovo clustering and directly provided a feature table and sequence as input and adjusted the similarity percentage.
Finally, it worked out.
Thanks so much for your input.
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