Clustering ASVs into OTUs

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1

Hi I am trying to cluster my ASVs into OTUs by following this Qiime Tutorial
https://docs.qiime2.org/2023.9/tutorials/otu-clustering/
I have tried adding my fasta file downloaded from rep-seq but I am getting this error

(1/1) Invalid value for '--i-sequences': Expected an artifact of at least
type SampleData[Sequences] | SampleData[SequencesWithQuality] |
SampleData[JoinedSequencesWithQuality]. An artifact of type
FeatureData[AlignedSequence] was provided.

Further I am not able to convert fasta file in fna for initially converting in into qza

Please assist me with this

Thanks

3

Hi @Vaishnavi_Dixit
Welcome to the :qiime2: forum!
Your issue is here:

You are providing aligned sequences and your command doesn't allow aligned sequences. Do you have any of these types: SampleData[Sequences] | SampleData[SequencesWithQuality] |
SampleData[JoinedSequencesWithQuality]?

Can you provide more info? What command are you running and what does your data look like?

5

Hey, Thanks for your help.

I used the code available for Denovo clustering and directly provided a feature table and sequence as input and adjusted the similarity percentage.

Finally, it worked out.

Thanks so much for your input.

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6

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