classify-consensus-blast -- BLAST6 file is empty

Hello, im using classify-consensus-blast for two paired end sample, but not running in my qiime.

Bellow is my syntax
qiime feature-classifier blast
--i-query vsearch-merged-rep-seqs-dada2-clustering.qza
--i-reference-taxonomy 18S_taxonomy.qza
--i-reference-reads 18S_.qza
--p-maxaccepts 1
--p-perc-identity 0.97
--p-query-cov 0.8
--o-classification taxonomy.qza
--o-search-results blast-search-results.qza

and i get massage from qiime like this

Plugin error from feature-classifier:

BLAST6 file is empty.

Debug info has been saved to /tmp/qiime2-q2cli-err-bpw8o51n.log

thank you

1 Like

Dear @Siti_Maysaroh ,

Welcome to the forum! This error message indicates that the BLAST6 output is empty, because no hits were found. You should check the query, reference database, and parameters to make sure that these settings are appropriate.

Good luck!