Hello, im using classify-consensus-blast for two paired end sample, but not running in my qiime.
Bellow is my syntax
qiime feature-classifier blast
--i-query vsearch-merged-rep-seqs-dada2-clustering.qza
--i-reference-taxonomy 18S_taxonomy.qza
--i-reference-reads 18S_.qza
--p-maxaccepts 1
--p-perc-identity 0.97
--p-query-cov 0.8
--o-classification taxonomy.qza
--o-search-results blast-search-results.qza
--verbose
and i get massage from qiime like this
Plugin error from feature-classifier:
BLAST6 file is empty.
Debug info has been saved to /tmp/qiime2-q2cli-err-bpw8o51n.log
thank you