I am trying to train my own classifier on QIIME2 with the SILVA database but ran into an importing issue. I tried importing silva_132_99_16S.fna from the SILVA database into qiime and I tried the following code which I got from here
#ran this first just to make sure all letters were capitalized as recommended in other post
cat silva132_99.fna | tr ‘acgt’ ‘ACGT’ > seqs.fna
qiime tools import
And I get the following error: seqs.fna is not a(n) QIIME1DemuxFormat file
I also tried adding in the --input-format QIIME1DemuxFormat with no avail.
I also tried this on the 16S only fna file from the SILVA 132 folder as well. I got the folder from here.
Any clues as to why this might be happening? I may simply resort to using your pre-trained classifier that you have. Is there any knowledge as to how much effectiveness you lose when using a pre-trained classifier versus training with your own sequences?
Thanks for the help!