can someone help me to decide in denoising this paired end sequence

[Mathi]

Hello
i am a beginer in using qiime2 and i have some doubt in it. can you suggest me on what basis i have to denoise this paired end
demux-paired.qzv (316.0 KB)
also my sole purpose of doing this is to perform a metagenome prediction in picrust. so do i have to rarefy or build relative abundance before implementing this feature table into picrust.(Note: i am using picrust in a new environment)

[colinbrislawn (Colin J Brislawn)]

Hello Mathi,

Welcome to the forums! :qiime2:

Thanks for posting the demux-paired.qzv file. The quality looks good and you are off to a great start!

I opened the .qzv file with httpw://view.qiime2.org and copied the image here:

How long is the amplicon you sequenced?

Have you tried running DADA2 already and what settings did you use?

[Mathi]

Thanks for your reply

is this is what you are asking and i had tried running dada2 with turncat len f = 264 and turncat len r = 192. i dont whether this is correct or wrong

[Mathi]

and this this the denoising stats i got after running dada2

[colinbrislawn (Colin J Brislawn)]

We have the data we need!

Use this post to estimate how long your amplicon will be, and how much overlap you will get with f=264 and r=192.
(This should explain why the 'percent of input merged' is <20%.)

Looks like you have two samples. Do you have more samples or are you just starting with two?

[Mathi]

thankyou for your help and yes for now i am starting with just two