Hello,
I am learning about PCR bias associated with the GC content of amplified bacterial DNA. It seems that the more GC content a target has the less it will be amplified in PCR. I was wondering how I might know the GC content of each bacterial group before beginning the PCR. Is there a way to calculate the GC content of V4 regions using QIIME2 and reference databases?
Some PCR master mixes supply GC enhancers to increase the representation of high GC targets. It could be useful to know the relative amount of high GC bacteria before I begin PCR so as to add the right about of the GC enhancer.
I'd be appreciative of anyone's thoughts on this :).